Prophylaxic study of Combination Ethyl Acetate Fraction of Andrographis paniculata Ness. and Dihidroartemisinin-Piperaquine (DHP) in Malarial Infected Mice

Abstrak

The resistance to some antimalarial drugs has had a major impact on the drug selection for malaria prophylaxis. According to previous research, the combination of Andrographis paniculata and Dihydroartemisinin-piperaquine (DHP) can prevent artemisinin resistance, reduce the adverse effects and is highly effective as an antimalarial. This study aims to determine the malaria prophylactic activity and survival time of the combination of A. paniculata ethyl acetate fraction with DHP on P. berghei infected mice. An in vivo malaria prophylaxis study was conducted based on Peters’ prophylactic test method. The combination of A. paniculata ethyl acetate fraction at a dose of 100 mg andrographolide/kg BW was administered twice a day per oral for four days and continued for another day after infection, meanwhile DHP at a dose 1,6 mg/kg BW was administered once a day per oral for three days. These results show that the combination of A. paniculata ethyl acetate fraction with DHP can inhibit 54,35% of the malaria parasite on day 4 and 44,98% on day 5, which was less active than the monotherapy group of A. paniculata ethyl acetate fraction or DHP alone. Also, this combination can increase the survival time of the mice to 6 days.